Methods for retrieval of sequence-verified DNA constructs

作者: Jacob Kitzman , Jay Shendure , Rupali Patwardhan , Jerrod Schwartz , Joseph Hiatt

DOI:

关键词:

摘要: In some embodiments, methods of recovering a sequence-verified target nucleic acid are provided. such may include tagging each member library with set adaptor sequences; sequencing the tagged members library; and from sequenced using dial-out selection method. certain be second sequences.

参考文章(20)
Peer Stähler, Raphael Carapito, Programmable oligonucleotide synthesis ,(2007)
George M. Church, Adeyemi Adesokan, Biodetection methods and compositions ,(2010)
Robi D. Mitra, George M. Church, Replica amplification of nucleic acid arrays ,(2000)
D. G. Gibson, J. I. Glass, C. Lartigue, V. N. Noskov, R.-Y. Chuang, M. A. Algire, G. A. Benders, M. G. Montague, L. Ma, M. M. Moodie, C. Merryman, S. Vashee, R. Krishnakumar, N. Assad-Garcia, C. Andrews-Pfannkoch, E. A. Denisova, L. Young, Z.-Q. Qi, T. H. Segall-Shapiro, C. H. Calvey, P. P. Parmar, C. A. Hutchison, H. O. Smith, J. C. Venter, Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome Science. ,vol. 329, pp. 52- 56 ,(2010) , 10.1126/SCIENCE.1190719
Brock F Binkowski, Kathryn E Richmond, James Kaysen, Michael R Sussman, Peter J Belshaw, Correcting errors in synthetic DNA through consensus shuffling Nucleic Acids Research. ,vol. 33, ,(2005) , 10.1093/NAR/GNI053
Karl Maurer, John Cooper, Marcelo Caraballo, James Crye, Dominic Suciu, Andrey Ghindilis, Joseph A. Leonetti, Wei Wang, Francis M. Rossi, Axel G. Stöver, Christopher Larson, Hetian Gao, Kilian Dill, Andy McShea, Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays PLOS ONE. ,vol. 1, ,(2006) , 10.1371/JOURNAL.PONE.0000034
D. G. Gibson, G. A. Benders, C. Andrews-Pfannkoch, E. A. Denisova, H. Baden-Tillson, J. Zaveri, T. B. Stockwell, A. Brownley, D. W. Thomas, M. A. Algire, C. Merryman, L. Young, V. N. Noskov, J. I. Glass, J. C. Venter, C. A. Hutchison, H. O. Smith, Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science. ,vol. 319, pp. 1215- 1220 ,(2008) , 10.1126/SCIENCE.1151721
Mark Matzas, Peer F Stähler, Nathalie Kefer, Nicole Siebelt, Valesca Boisguérin, Jack T Leonard, Andreas Keller, Cord F Stähler, Pamela Häberle, Baback Gharizadeh, Farbod Babrzadeh, George M Church, High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing Nature Biotechnology. ,vol. 28, pp. 1291- 1294 ,(2010) , 10.1038/NBT.1710
Joseph B Hiatt, Rupali P Patwardhan, Emily H Turner, Choli Lee, Jay Shendure, Parallel, tag-directed assembly of locally derived short sequence reads Nature Methods. ,vol. 7, pp. 119- 122 ,(2010) , 10.1038/NMETH.1416
J. Smith, P. Modrich, Removal of polymerase-produced mutant sequences from PCR products Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 6847- 6850 ,(1997) , 10.1073/PNAS.94.13.6847