Analysis of the duplicated CHS1 gene related to the suppression of the seed coat pigmentation in yellow soybeans.
作者： M. Senda , A. Jumonji , S. Yumoto , R. Ishikawa , T. Harada
DOI: 10.1007/S00122-001-0801-4
关键词:
摘要: Seed coat color in soybean is controlled by the classically defined I (Inhibitor) locus. The seeds of most commercial varieties are yellow due to presence a dominant allele locus (I: seed coat, or i i : pigmented hilum and coat), which inhibits pigmentation. Analysis spontaneous mutations from (yellow coat) i (pigmented has shown that these correlated with deletion duplicated chalcone synthase gene-1 (CHS1) region. In current study, we isolated CHS1 region cultivar I/I genotype (cv Miyagi shirome) determined its structure. results showed contained intact regulatory coding regions. We designated as ICHS1. hypocotyls shirome, cDNA derived ICHS1 mRNA was identified reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, whereas immature coats it suggested amount transcripts and/or another type (CHS1.1) very low. Interestingly, shirome genome genotype, closely linked truncated CHS3, sequence comparison this cluster probably arose CHS1–CHS3 1.8-kb event.
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