Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
作者： George Georgiou , James R Swartz , Paul Bessette , Ji Qiu
DOI:
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摘要: Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments, the involve co-expression of a prokaryotic isomerase, such as DsbC or DsbG gene encoding recombinant eukaryotic polypeptide. Exemplary disclosed include tissue plasminogen activator.
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