Quantitative Detection and Differentiation of Free-Living Amoeba Species Using SYBR Green–Based Real-Time PCR Melting Curve Analysis
作者： Jonas Behets , Priscilla Declerck , Yasmine Delaedt , Lieve Verelst , Frans Ollevier
DOI: 10.1007/S00284-006-0241-0
关键词:
摘要: Real-time polymerase chain reaction melting curve analysis (MCA) allows differentiation of several free-living amoebae species. Distinctive characteristics were found for Naegleria fowleri, N. lovaniensis, australiensis, gruberi, Hartmanella vermiformis, and Willaertia magna. Species specificity the amplicons was confirmed using agarose gel electrophoresis sequence-based approaches. Amplification efficiency ranged from 91% to 98%, indicating quantitative potential assay. This MCA approach can be used detection after cultivation but also as a culture-independent method.
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