Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma.

摘要: An epithelial cell line, MDA-MB-157, has been established from the pleural fluid of a metastatic human breast carcinoma. The line is characterized by rapid growth cells in dense plaques and suspension. were originally isolated complex medium containing Leibovitz's L-15, Waymouth's MB752/1 Ham's F-10 media with 16% fetal calf serum, glutathione, cortisol, insulin, antibiotics. Recently cultures have growing equally well on some less such as RPMI-1640 20% serum or serum. presence typical organization, structures desmosomes, observed electron microscopy. Viruses mycoplasmas not detected. exhibit hypotriploid karyotype mode 64 to 66 chromosomes.