Experimental autoimmune dacryoadenitis: purification and characterization of a lacrimal gland antigen.
作者： S. H. Liu , De-Hu Zhou
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摘要: A soluble lacrimal gland antigen (LG-Ag) capable of inducing experimental autoimmune dacryoadenitis (EAD) in SJL/J mice has been purified from bovine extracts by a combination ion exchange and gel-filtration chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed single band with mobility corresponding to molecular weight 45,000 daltons. Antibodies raised guinea pigs against LG-Ag were used confirm the tissue-specificity assess cellular localization within tissues. Anti-LG-Ag serum reacted crude but not submaxillary gland, parotid or retina. As shown immunofluorescent staining, appeared be localized on murine ductal epithelial cells was lacking salivary ducts. induced EAD that characterized infiltration mononuclear around ducts associated vasculature, accompanied extensive damage acinar cells. The lesions tissue-specific, immunized had intense lymphocytic infiltrates no significant their harderian glands. availability LG can facilitate further analysis pathogenetic mechanisms EAD.
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R. B. Goudie, K. Whaley, J. Williamson, W. W. Downie, D. M. Chisholm, J. A. Boyle, W. C. Dick, Salivary duct autoantibody in Sjøgren's syndrome: correlation with focal sialadenitis in the labial mucosa Clinical and Experimental Immunology. ,vol. 4, pp. 273- ,(1969)
N Talal, T M Tarpley, G L Schall, R O Wolf, N A Cummings, L G Anderson, Cellular-versus-humoral autoimmune responses to salivary gland in Sjögren's syndrome. Clinical and Experimental Immunology. ,vol. 13, pp. 335- 342 ,(1973)
Igal Gery, Toichiro Kuwabara, Robert B. Nussenblatt, Dean D. Metcalfe, Chi-Chao Chan, Manabu Mochizuki, An association between susceptibility to experimental autoimmune uveitis and choroidal mast cell numbers. Journal of Immunology. ,vol. 133, pp. 1699- 1701 ,(1984)
R A Prendergast, A M Silverstein, S H Liu, Experimental autoimmune dacryoadenitis. II. Harderian gland disease in the rat. Investigative Ophthalmology & Visual Science. ,vol. 28, pp. 276- 280 ,(1987)
D.Scott Linthicum, John J. Munoz, Alan Blaskett, Acute experimental autoimmune encephalomyelitis in mice: I. Adjuvant action of Bordetella pertussis is due to vasoactive amine sensitization and increased vascular permeability of the central nervous system Cellular Immunology. ,vol. 73, pp. 299- 310 ,(1982) , 10.1016/0008-8749(82)90457-9
I. Gery, M. Mochizuki, R.B. Nussenblatt, Chapter 3 Retinal specific antigens and immunopathogenic processes they provoke Progress in Retinal Research. ,vol. 5, pp. 75- 109 ,(1986) , 10.1016/0278-4327(86)90006-4
Yehuda Shoenfeld, David A. Isenberg, The mosaic of autoimmunity Immunology Today. ,vol. 10, pp. 123- 126 ,(1989) , 10.1016/0167-5699(89)90245-4
L.D. Lise, F. Audibert, Immunoadjuvants and analogs of immunomodulatory bacterial structures Current Opinion in Immunology. ,vol. 2, pp. 269- 274 ,(1989) , 10.1016/0952-7915(89)90199-4
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0
T. E. W. Feltkamp, A. L. van Rossum, Antibodies to salivary duct cells, and other autoantibodies, in patients with Sjögren's syndrome and other idiopathic autoimmune diseases. Clinical and Experimental Immunology. ,vol. 3, pp. 1- 16 ,(1968)