Broad Spectrum Peptide Vaccine Design Against Hepatitis C Virus

摘要: Background Hepatitis C virus (HCV) infection is a global burden. There no peptide vaccine found as modality to cure the disease available due weak cellular immune response and limitation induce humoral response. Methods Five predominated HCV subtypes in Indonesia (1a, 1b, 1c, 3a, 3k) were aligned conserved regions selected. Twenty alleles of class I MHC including HLA-A, HLA-B, HLAC types used predict potential epitopes by using NetMHCPan IEDB. Eight HLA-DRB1, together with combination 3 HLA-DQA1 5 HLA-DQB1 utilized for Class II prediction NetMHCIIPan LBtope Ig- Pred B cells epitopes. Moreover, proteasome analysis was performed NetCTL stability HLA calculated NetMHCStabPan I. All predicted analyzed its antigenicity, toxicity, stability. Population coverage, molecular docking dynamics several best Results The results showed that two from envelop protein, GHRMAWDMMMNWSP (E1) PALSTGLIHLHQN (E2) selected promising cell CD8+ T inducers. Other peptides, LGIGTVLDQAETAG VLVLNPSVAATLGF, taken NS3 protein CD4+ inducer. Conclusion This study suggested utilization all four peptides make combinational vivo prove ability inducing secondary toward HCV.