Improved method for simultaneous determination of ascorbic acid and dehydroascorbic acid, isoascorbic acid and dehydroisoascorbic acid in food and biological samples
作者： Morten A Kall , Carina Andersen
DOI: 10.1016/S0378-4347(99)00193-0
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摘要: The total vitamin C amount in different food and plasma samples was determined by a dual detection system, after HPLC separation, with direct of ascorbic acid indirect fluorimetric dehydroascorbic post-column O-phenyldiamine derivatisation. two active forms their D-isomers were separated within 10 min. repeatability measurement several fruits vegetables ranged from 0.3 to 1.9% (relative standard deviation) for C. reproducibility, based on double determinations, 1.9 3.6% C, depending the matrix. determinations reference materials, 2.4 3.7% 4.3 5.8% acid, again
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