Separation and quantitative determination of galactosamine and glucosamine at the nanogram level by sulphonyl chloride reaction and high-performance liquid chromatography
作者： A. Hjerpe , C.A. Antonopoulos , B. Classon , B. Engfeldt
DOI: 10.1016/S0021-9673(00)91831-4
关键词:
摘要: Abstract Two chromatographical systems for the separation and determination of glucosamine galactosamine are described. In more rapid less sensitive two systems, hexosamines treated with toluene sulphonyl chloride subsequently separated in a reversed-phase system. this mode 0.4–50 μg hexosamine chromatographed within 10 min. second system, Dns-Cl similar column. With method, 0.02–2 is possible 25 When internal standard was used, procedure lengthened by ca . Both determinations can also be used when there large differences relative amounts hexosamines.
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