Chapter 15 Pre- and Post-Column Detection-Oriented Derivatization Techniques in HPLC of Carbohydrates
作者： Sumihiro Hase
DOI: 10.1016/S0301-4770(08)60520-5
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摘要: Publisher Summary This chapter discusses pre- and post-column detection-oriented derivatization techniques used in high-performance liquid chromatography (HPLC) of carbohydrates. Carbohydrates must be purified with high sensitivity resolution for structural elucidation further use as ligands lectins immunoglobulins, enzyme substrates, receptors. A chemical method allows the conversion carbohydrates into derivatives that can detected higher sensitivity. Also, often enhances selectivity detection, because only reducing sugars modified presence amino acids lipids when carbonyl groups are derivatized. Post-column has advantage analyzed without any modification. The basically uses color reactions consume part effluents detection after requires a postcolumn reactor combined column chromatography. Pre-column also serve some additional advantages—for example, (1) enables sugar mapping by HPLC, which is convenient assessing gross structures detecting alterations chains derived from glycoproteins, (2) additivity rule found between elution times case reversed-phase HPLC pyridylaminated chains.
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