Identification of aTheileria annulataAntigen Expressed in Multiple Stages of the Parasite Life Cycle

摘要: Abstract Knight, P. A., Williamson, S. W., Brown, C. G. D., Bell-Sakyi, L., Kirvar, E., Kinnaird, J., Hussain, K., Hall, R., Boulter, N., and Tait, A. 1998. Identification of aTheileria annulataantigen expressed in multiple stages the parasite life cycle.Experimental Parasitology90, 110–121. In order to identify sporozoite surface molecules which may be involved invasion could act as potential vaccine candidates, a number Mabs were raised mice againstT. annulatasporozoites. These assayed for their ability block bovine peripheral blood mononuclear (PBM) cellsin vitro.One these, Mab 4B11, was found neutralize high degree recognize group antigens on Western blots. AT. annulataλgt11 genomic expression library screened with 4B11 positive clone containing 900-bp insert (KP8) analysed further. Data from Southern Northern blotting indicated that gene KP8 sequence, termedsporozoite andmacroschizont 2 (spm2), both inT. annulatasporozoites later life-cycle stages, macroschizont-infected leucocytes piroplasms. The sequence inE. colias fusion protein glutathione-S-transferase (GST) using vector pGEX1λT. Bovine antiserum against GST-KP8 recognised single molecular weight molecule blots corresponding one by sporozoites, leucocytes, While our evidence suggests spm2 alone is not responsible neutralization, it multistage antigen likely function subsequent stages.