Observations on the separation of Theileria sporozoites from ticks
作者： A.R. Walker , S.B. McKellar
DOI: 10.1016/0020-7519(83)90044-9
关键词: Differential centrifugation 、 Density gradient 、 Centrifugation 、 Percoll 、 Virology 、 Filtration 、 Biology 、 Theileria 、 Suspension (chemistry) 、 Chromatography 、 Giemsa stain
摘要: Abstract Hyalomma anatolicum ticks infected with Theileria annulata were partially fed on rabbits and then ground up tissue culture medium. The treated by centrifugation at 100 g , filtration through membranes of 8 μm pore diameter a discontinuous density gradient Percoll. Counts sporozoites tick debris made from Giemsa stained slides samples each stage the separation. Debris was removed during light greater rate than sporozoites. After approximately 41% original remained in suspension. most found distinct zone, approx. 1·08 g/cm 3 density, separate dense soluble contaminants. this final 24% recovered
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