Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences.

摘要: Epithelial cells at all mucosal surfaces are potentially apposed to bacteria, particularly in the intestine. It is established that intestinal epithelial (IECs) represent an important barrier between lamina propria and harmful lumenal contents. In addition, IECs immunoeffector with capacity release cytokines, chemokines, other molecules involved antigen presentation immune defense. The interaction of bacteria can result a decrease function development inflammation, which known be factor pathology. potential role such crosstalk cell types normal physiology and/or pathophysiology therefore topic intense investigation. this chapter, we provide protocols for identification associated epithelium mucosa addition functional assays examining interactions neutrophils cell-mediated killing bacteria.