Rapid detection of virus genome from imported dengue fever and dengue hemorrhagie fever patients by direct polymerase chain reaction
作者： Kouichi Morita , Tatsuo Maemoto , Shoji Honda , Kenji Onishi , Misako Murata
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摘要: Serum specimens from patients with imported dengue fever and hemorrhagic were directly subjected to reverse transcription polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. results of PCR corresponded closely the isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis infection. © 1994 Wiley-Liss, Inc.
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