Inhibition of Growth of Human Breast Cancer Cell Line MCF-7 by Serum Derived From Calcium Chloride-Clotted Plasma

摘要: The growth of MCF-7 cells, established from a metastatic mammary carcinoma, and HBL-100 derived primary culture human milk, was examined in medium supplemented with whole blood serum (WBS), defibrinogenated plasma, plasma-derived (PDS). PDS obtained platelet-poor plasma collected the anticoagulant citrate phosphate dextrose clotted by addition calcium chloride did not promote cells as well WBS or alone. Growth-inhibitory activity observed when final cell densities presence combined fetal bovine (FBS) were compared those control cultures maintained FBS level this varied among donors. Inhibition cells. Calcium induce inhibitory added to platelet components alter inhibition PDS. However, extracts affect expression before clotting. Activity diminished following dialysis PDS, which suggested that stemmed small-molecular-weight factor for depended on mechanism coagulation.