Molecular ecology of two commercially important crustacean species, Nephrops norvegicus and Macrobrachium rosenbergii: Implications for the management of fisheries and aquaculture

摘要: Molecular ecology is one of the most important branches evolutionary biology, and it uses advantages molecular techniques such as PCR-RFLP, sequencing, microsatellite analysis, recently introduction next generation to address outstanding issues in fields population genetics phylogeny. The genomic approach has been influential providing new information relevant traditional questions ecology, genetic differentiation, speciation, species adaptation others. rationale present thesis was incorporate both PCR-RFLP sequencing gain on variability two commercially-important crustacean species, namely Norway lobster Nephrops norvegicus giant freshwater prawn Macrobrachium rosenbergii. Nephrops exhibits large morphological even between adjacent populations. first objective this investigate N. from three localities, Clyde Sea area North Minch (North Sea) well Icelandic waters. phylogeographic study found no significant differentiation populations studied areas (FST: 0.01819). This finding consistent with outcomes previous studies that were not geographically structured. Outcomes strongly suggest environmental factors, rather than are more likely play a role high observed species. A rosenbergii then undertaken contribution understanding complex biogeography world, Indo Australian Archipelago (IAA). IAA incredible richness endemism location 4 out 25 world’s biodiversity hotspots, Sundaland, Philippines, Indonesia Wallacea. Within Wallace’s line Huxley’s line, abrupt faunal transition world lies Sunda Sahul shelves. used thesis, M. an ideal model wide geographical distribution across IAA. The screened COI segment using technique collected eight locations Malaysia peninsular east Malaysia. These exhibited 0.62503) mainly due sample Sabah. However, (Sarawak) similar Peninsular Malaysia, though Northern Sarawak showed sub-population main cluster (cluster I) indicating diversity restricted. Beside, II indicated confirmed recent aquaculture activities restocking Kedah, Perak Knowledge levels could assist management be managed stock conservation recovery programme carried based knowledge all lack within In contrast, possessed level have separately, especially for unique also useful future research wild population, product improvement purposes. Sabah potentially programmes. One aspects see whether resistant disease infection. A preliminary conducted susceptibility Malaysian infection by human food-poisoning bacterium V. parahaemolyticus. Nonetheless, virulence stage bacterial strain, status immune system host, size age experimental animals dose injected bacteria might contributed inconclusiveness results. principle screening resistance sound, may lead improvements quality broodstock industry.