Characterization, sub-cellular localization and expression profiling of the isoprenylcysteine methylesterase gene family in Arabidopsis thaliana

摘要: Background: Isoprenylcysteine methylesterases (ICME) demethylate prenylated protein in eukaryotic cell. Until now, knowledge about their molecular information, localization and expression pattern is largely unavailable plant species. One ICME Arabidopsis, encoded by At5g15860, has been identified recently. Over-expression of At5g15860 caused an ABA hypersensitive phenotype transgenic Arabidopsis plants, indicating that it functions as a positive regulator signaling. Moreover, induced the this gene seedlings. The current study extends these findings examining sub-cellular localization, profiling, physiological two other ICME-like proteins, ICME-LIKE1 ICME-LIKE2, which were related genes At1g26120 At3g02410, respectively. Results: Bioinformatics investigations showed homologs comprise small subfamily carboxylesterase (EC 3.1.1.1) Arabidopsis. Sub-cellular GFP tagged its proteins are intramembrane predominantly localizing endoplasmic reticulum (ER) Golgi apparatus. Semi-quantitative real-time quantitative PCR revealed expressed all examined tissues, including roots, rosette leaves, cauline stems, flowers, siliques, with differential levels. Within family, base transcript abundance ICME-LIKE2 very low higher reproductive organs (flowers siliques). Time-course analysis uncovered both up-regulated mannitol, NaCl treatment, showing highest level up-regulation treatments. Heat stress resulted significantly but down-regulation genes. Cold dehydration stimuli led to no significant change expression. Mutant icme-like2-1 increased sensitivity slightly decreased salt osmotic stresses during seed germination. Conclusions: It concluded family involved signaling probably through mediating process demethylating proteins. Identification will help better understand significance prenylation Planta.