Extending the linear range for kinetic reactions by considering the relationship between enzyme activity of the reagent and measurement intervals.

摘要: We show that the rate-concentration curve is sigmoidal for enzyme-catalyzed procedures are commonly applied to rapid automated analyzers. Linear data can be obtained by judicious selection of reagent enzyme activity (Vm) and measurement interval (t1 t2). For determination substrate concentrations much less than Km, conditions balance linearity with sensitivity accuracy when Vm/Km = [ln (t2/t1)]/(t2-t1). also present theoretical experimental linear range extended exceeding Km value. illustrate application theoretically appropriate analysis reported optimized. Familiarity these concepts obviate laborious potentially misleading experimentation.