Determination of enzyme substrates with an extended range of linearity

摘要: Abstract This paper examines the possibility of using a one-component kinetic model for determination l -amino acids having concentrations near or greater than their Michaelis constants. A commercial nonlinear regression program is used to fit data absorbance and rate vs. time Michaelis-Menten equation. Coupled acid oxidase reactions are estimate such parameters as maximum velocity, V m , constant, K forward k 1 . conventional mixing method an air-segmented continuous flow (ASCF)/stopped-flow applied monitor course reaction. The correlation results obtained between two methods studied. accuracy precision analytical by proposed reported compared those from double reciprocal plots.