In vivo fluorometric method for early detection of cyanobacterial waterblooms
作者： Tsaiyun Lee , Mikio Tsuzuki , Toshifumi Takeuchi , Kenji Yokoyama , Isao Karube
DOI: 10.1007/BF02182403
关键词:
摘要: A specific method was developed for monitoring the concentration of cyanobacteria (blue-green algae) before waterblooms, based on their characteristics ofin vivo fluorescence. The excitation and emission spectra are very different from those eukaryotic algae, due to importance phycocyanin, rather than chlorophylla, in determining fluorescence characteristics. Our results, four cyanobacteria:Microcystis aeruginosa, Anabaena cylindrica, Phormidium tenue andSpirulina platensis, indicate that at 620 nm its 645 is a sensitive detection. Furthermore, addition 10 μM photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) induced only 3% increase phycocyanin fluorescence, suggesting this measurement almost independent ongoing rate photosynthesis.
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