Development of a fluorometric sensor for the measurement of phycobilin pigment and application to freshwater phytoplankton

摘要: A two-channel fluorometric sensor system for the detection of waterbloom phytoplankton Microcystis aeruginosa has been developed. Excitation wavelengths 620 and 440 nm were used, former detecting cyanobacteria themselves, latter subtracting interference due to eukaryotic algae present in sample. The fluorescence was measured at 645 680 nm, respectively. linear relationship between phycocyanin established species examined range 10−4 100 g/ml−1 chlorophyll a. Furthermore, upon ultrasonic treatment a colony cyanobacteria, intensity cell concentration improved. Applied real samples Lake Kasumigaura, Japan, after ultrasonication, this method could detect waterbloom. This is capable rapid determination phycocyanin; time required one measurement cycle approximately 25 min including sonication rinsing 5 min. limit suitable early stage formation. device based on used continuous situ monitoring lake water. © 2000 John Wiley & Sons, Inc. Field Analyt Chem Technol 4: 53–61,