Cationic liposome-mediated enhanced generation of human HLA-restricted RSV-specific CD8+ CTL+.
作者： Innocent N. Mbawuike , Yongxin Zhang , Ying Wang , Ling Song
关键词:
摘要: Generation of human CD8+ cytotoxic T-lymphocyte (CTL) activity against respiratory syncytial virus (RSV) using peripheral blood leukocytes (PBL) in vitro is inefficient. Lipofectamine, a polycationic liposome, previously shown to enhance the transfection efficiency DNA cells, was evaluated for enhancing RSV CTL activity. Stimulator cells were prepared by infecting PBL with or without Lipofectamine 3 hr and then transferred responder cells. After 8 days incubation, lysis autologous target infected (also treated Lipofectamine) determined 4-hr 51chromium release assay. treatment significantly enhanced HLA-restricted RSV-specific (up sevenfold, P < 0.05–0.001). also cell surface antigen expression increased frequencies HLA-A,B,C+/RSV+ HLA-DR+/RSV+ as demonstrated flow cytometry. These results demonstrate usefulness cationic liposomes augmenting increasing generation
springer.com
sci-hub.se 参考文章(58)
W. Paul Glezen, Reactive airway disorders in children. Role of respiratory virus infections. Clinics in Chest Medicine. ,vol. 5, pp. 635- 643 ,(1984) , 10.1016/S0272-5231(21)00289-6
XIAO-LING LI, MADANMOHAN BOYANAPALLI, XIAO WEIHUA, DHANANJAYA V. KALVAKOLANU, BRET A. HASSEL, Induction of interferon synthesis and activation of interferon-stimulated genes by liposomal transfection reagents. Journal of Interferon and Cytokine Research. ,vol. 18, pp. 947- 952 ,(1998) , 10.1089/JIR.1998.18.947
Rol.M. Zinkernagel, Pete.C. Doherty, MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Advances in Immunology. ,vol. 27, pp. 51- 177 ,(1979) , 10.1016/S0065-2776(08)60262-X
C. R. M. Bangham, P. J. M. Openshaw, L. A. Ball, A. M. Q. King, G. W. Wertz, B. A. Askonas, Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants. Journal of Immunology. ,vol. 137, pp. 3973- 3977 ,(1986)
Innocent N Mbawuike, Catherine L Acuna, Kirsten C Walz, Robert L Atmar, Stephen B Greenberg, Robert B Couch, Cytokines and impaired CD8+ CTL activity among elderly persons and the enhancing effect of IL-12 Mechanisms of Ageing and Development. ,vol. 94, pp. 25- 39 ,(1997) , 10.1016/S0047-6374(96)01855-6
B. T. Rouse, S. Nair, R. Reddy, L. Huang, Fan Zhou, In vivo cytotoxic T lymphocyte induction with soluble proteins administered in liposomes. Journal of Immunology. ,vol. 148, pp. 1585- 1589 ,(1992)
J A Nicholas, K L Rubino, M E Levely, E G Adams, P L Collins, Cytolytic T-lymphocyte responses to respiratory syncytial virus: effector cell phenotype and target proteins. Journal of Virology. ,vol. 64, pp. 4232- 4241 ,(1990) , 10.1128/JVI.64.9.4232-4241.1990
A W Paton, J C Paton, A J Lawrence, P N Goldwater, R J Harris, Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification. Journal of Clinical Microbiology. ,vol. 30, pp. 901- 904 ,(1992) , 10.1128/JCM.30.4.901-904.1992
A H Cherrie, K Anderson, G W Wertz, P J Openshaw, Human cytotoxic T cells stimulated by antigen on dendritic cells recognize the N, SH, F, M, 22K, and 1b proteins of respiratory syncytial virus. Journal of Virology. ,vol. 66, pp. 2102- 2110 ,(1992) , 10.1128/JVI.66.4.2102-2110.1992
Barry T. Rouse, Leaf Huang, Leaf Huang, Fan Zhou, Induction of cytotoxic T lymphocytes in vivo with protein antigen entrapped in membranous vehicles. Journal of Immunology. ,vol. 149, pp. 1599- 1604 ,(1992)