Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification.

摘要: A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific the region RSV genome which encodes F1 subunit fusion (F) glycoprotein. Other viruses do not give positive reaction. was tested on 202 aspirates collected from children with clinical signs infection, results were compared those obtained culture direct by enzyme immunoassay (EIA). 118 125 samples cultured, as well 4 7 negative but EIA positive. 68 70 culture-negative, EIA-negative samples, included 11 other isolated. speed, sensitivity (94.6%), specificity (greater than 97%) suggest that this technique could be useful samples.