Identifying bovine respiratory syncytial virus by reverse transcription-polymerase chain reaction and oligonucleotide hybridizations.

摘要: An assay to identify tissue culture cells infected with bovine respiratory syncytial virus (BRSV) that utilizes reverse transcription (RT), the polymerase chain reaction (PCR), and a synthetic oligonucleotide hybridization probe has been developed. The RT-PCR uses BRSV-specific negative-sense primer synthesize cDNA from BRSV fusion protein mRNA template another (positive sense) upstream for PCR amplification. In presence of templates isolates originating locations throughout United States, resulted in amplified products (381 bp) were specific BRSV, as demonstrated hybridizations positive-sense complementary internal sequences sequence comparisons F 391-2. analysis prototype strains human RSV subgroups A B, amplification similar 381-bp product was not evident, no hybridized probe. We conclude ability amplify DNA by then demonstrate will greatly add speed, sensitivity, specificity diagnostics.