Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis.
作者： Marie Cariou , Céline Ribière , Stéphanie Morlière , Jean-Pierre Gauthier , Jean-Christophe Simon
DOI: 10.1186/S13104-018-3559-3
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摘要: Targeted sequencing of 16S rDNA amplicons is routinely used for microbial community profiling but this method suffers several limitations such as bias affinity universal primers and short read size. Gene capture by hybridization represents a promising alternative. Here we metagenomic extract from the pea aphid Acyrthosiphon pisum to compare performances two widely PCR primer pairs with DNA capture, based on solution hybrid selection. All methods produced an exhaustive description 8 bacterial taxa known be present in sample. In addition, yielded similar quantitative results, number reads strongly correlating controls. Both can thus considered qualitatively quantitatively robust sample low complexity.
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