A Guide to Carrying Out a Phylogenomic Target Sequence Capture Project.

摘要: High-throughput DNA sequencing techniques enable time- and cost-effective of large portions the genome. Instead annotating whole genomes, many phylogenetic studies focus effort on sets pre-selected loci, which further reduces costs bioinformatic challenges while increasing coverage. One common approach that enriches loci before is often referred to as target sequence capture. This technique has been shown be applicable greatly varying evolutionary depth. Moreover, it proven produce powerful, multi-locus datasets suitable for analyses. However, capture requires careful considerations, may affect success experiments. Here we provide a simple flowchart designing phylogenomic We discuss necessary decisions from identification final processing data. outline solutions related taxonomic scope, sample quality, available genomic resources projects. hope this review will serve useful roadmap carrying out successful studies.