Leucine and Calcium Regulate Fat Metabolism and Energy Partitioning in Murine Adipocytes and Muscle Cells

摘要: Dietary calcium modulation of adiposity is mediated, in part, by suppression calcitriol, while the additional effect dairy products mediated components; these include high concentration leucine, a key factor regulation muscle protein turnover. We investigated calcitriol and on energy metabolism murine adipocytes cells partitioning between skeletal muscle. Leucine induced marked increase fatty acid oxidation C2C12 (P < 0.001) decreased FAS expression 66% 3T3-L1 adipocytes. Calcitriol cell 37% increased adipocyte gene threefold 0.05); effects were partially reversed either leucine or channel antagonism with nifedipine. Co-culture incubation 48-h conditioned medium 62% 0.001), but treating and/or nifedipine attenuated this effect. Leucine, also modulated adiponectin production thereby exerted indirect myotubes. Adiponectin IL-15 IL-6 release myotubes inhibitory calcitriol. Comparable found treated derived from co-cultured These data suggest that promote to cells, resulting storage increasing utilization