Identification of the testis-specific protein 10 (TSGA10) as serologically defined tumour-associated antigen in primary cutaneous T-cell lymphoma.

摘要: Summary Background  The number of identified tumour-associated antigens for cutaneous lymphoma is still very restricted, which limits the elucidation tumour immunology these malignancies and development specific immunotherapies immunodiagnostics. Objectives  To identify new serologically defined associated with lymphoma. Methods  A phage expression library human testis transcriptom was established immunoscreened sera from 100 patients nine parapsoriasis, 81 age-matched control donors. Positive clones were sequenced to respective antigen. Results  testis-specific protein 10 (TSGA10) as an antigen recognized by two Mycosis fungoides but not healthy By reverse transcription–polymerase chain reaction analysis, TSGA10 found expressed in all samples tested, various cell lines, testis, peripheral blood mononuclear cells, skin, isolated lymphocytes, keratinocytes fibroblasts. overexpression had previously been reported other cancers. Conclusions  a lymphoma.