Human and animal microbial source tracking in a tropical river with multiple land use activities.

摘要: The enhancement and restoration of the water quality deteriorating surface resources can be challenging, particularly for rivers with multiple usages, such as agriculture, animal husbandry, human residence, industries. Recently, performance DNA-based microbial source tracking (MST) indicators detected by end-point quantitative PCR assays identifying sources fecal pollution from sewage, swine, cattle non-host-specific (universal) in Tha Chin River basin, Thailand, was evaluated. present study monitored these validated MST markers various physicochemical parameters samples collected twelve stations along during four sampling events wet dry seasons. No significant difference precipitation observed between samplings. Universal (both qPCR) were all 48 samples, indicating persistent continuing contamination. sewage- swine-specific qPCR marker concentrations did not vary among events, whereas cattle-specific only season. Animal-specific lower section, which is characterized intensive farming. Sewage-specific also found section near an upstream residential area. high agreement (87.5-100%) results suggested that could serve a lower-cost screening test requires less technical expertise. A multivariate analysis conducted using survival procedure to include censored data emphasized river at events. showed moderate correlations indicators, including total coliforms, E. coli, enterococci. None or associated measured parameters. This provides first evaluation monitoring freshwater findings might aid surveillance impaired bodies development strategies improving their quality.