Eicosanoids in the Phytophthora infestans-potato interaction: lipoxygenase metabolism of arachidonic acid and biological activities of selected lipoxygenase products

摘要: Potato tuber and leaf discs were treated with the fungal elicitor arachidonic acid. Hydroperoxy-eicosatetraenoic acids (HpETEs), produced by reaction of lipoxygenase acid, then extracted from tissues analysed. 5-, 8-, 11-, 12-, 15-HpETE formed rapidly both tissues. 5-HpETE was predominant product in discs, whereas 12- principal products discs. HpETEs within 10 min after addition increased during first 5 h treatment. The quantity HpETEs, however, inversely related to amount acid initially applied sesquiterpene phytoalexin levels induced. Treatments that enhance ( β -glucans Phytophthora infestans ) or inhibit (abscisic acid) activity generally did not have a corresponding effect on tissues, suggesting these treatments primarily affect response at sites different initial Eicosatetraynoic an acetylenic analogue irreversible inhibitor lipoxygenases, suppressed elicitor-induced hypersensitive inhibited formation microsomes A collection purified tested for potato fungitoxicity P. capsici . None possessed but several hydroxy-acids inhibitory cystospore germination. results are discussed relation proposed role hypersensitivity expression disease resistance potato.