Nucleic acid amplification using non-random primers

作者: Christopher K. Raymond , John Castle , Christopher Armour

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摘要: The present invention provides methods for selectively amplifying a target population of nucleic acid molecules (e.g., all mRNA expressed in cell type except the most highly species). also populations oligonucleotides including sequences set forth SEQ ID NOS: 1-933. These can be used, example, to prime synthesis cDNA complementary isolated from mammalian blood without priming globin mRNA, or ribosomal RNA molecules.

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