Electroosmotic pump‐supported molecularly imprinted monolithic column for capillary chromatographic separation of nitrophenol isomers

摘要: In this work, a novel molecularly imprinted polymer (MIP) monolithic column with integrated in-column electroosmotic pump (EOP) was designed and successfully prepared to facilitate the capillary chromatography MIP column. A silica-based EOP synthesized at detection end of by so-gel provide hydrodynamic driven force for chromatography. Because large surface area low fluidic resistance silica monolith,a strong steady EOF generated EOP, indicating that quite compatible With sufficient provided electro-driven based chromatographic separation nitrophenol isomers achieved in 4-vinylpyridine-based capillary, which originally proved infeasible because shortage. No significant influence upon specific recognition found due setting after window The experimental parameters on such as voltage pH value running buffer investigated. also evaluated liquid mode compare EOP-driven Higher efficiency obtained improved peak shape. results suggested EOP-supported technique would be good way solve problem weak generation