Very high-pressure capillary liquid chromatography assisted by voltage.

摘要: Capillary liquid chromatography at moderately high pressures and capillary electrochromatography (CEC) have been combined to drive the mobile phase through columns packed with small diameter particles. In a column 1.5 μm nonporous particles, linear velocities near 3 mm/s were observed when combining inlet of 690 bar (10,000 psi) an applied voltage 25 kV. Optimum velocity for was achieved using pressure-voltage combination 350 (5000 5 Separation efficiencies optimum agreed those predicted by van Deemter equation chromatography. Retention factors decrease under as increased; such behavior has attributed Joule heating effects.