Gene Therapy by Intramuscular Injection of Plasmid DNA: Studies on Firefly Luciferase Gene Expression in Mice
作者： Marston Manthorpe , Francine Cornefert-Jensen , Jukka Hartikka , Jiin Felgner , Ann Rundell
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摘要: ABSTRACT Direct injection of nonviral, covalently closed circular plasmid DNA into muscle results in expression the myofiber cells. We have examined firefly luciferase constructs injected adult murine skeletal muscle. Considerable variation enzyme was noted among with different regulatory elements, batches same construct, and similar transfection experiments performed at times. This minimized by using single performing comparable sets concurrently. A quantitative experimental protocol defined for comparing various aspects process. report that a construct containing human cytomegalovirus immediate-early gene promoter plus intron (a termed “p-CMVint-lux”) showed highest several tested. Dose–response time course analyses p-CMVint-lux injecti...
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