Immunocytochemical Studies of Neurofibrillary Tangles

摘要: The molecular nature of neurofibrillary tangles senile dementia the Alzheimer type (SDAT) was studied by immunoperoxidase and immunofluorescence techniques. Five antiserums, including anti-humanbrain-2-cycle-purified-microtubule-fractions (2 x MT), anti-calf-brain-2 MT, anti-sea-urchin-egg-tubulin, antibeef-brain-tubulin, anti-human-brain-neurofilament(NF)-210-kilodalton(kd)-protein were tested for their binding to tangles. antihuman-2 MT serum stained structures resembling tangles, neurites neuritic plaques, microglialike cells in SDAT brains, but no such staining pattern detected normal brain sections. In neurons isolated from about 40% labeled anti-human-2xMT with an identical pattern. Other antiserums did not preferentially bind tanglelike tissue sections bound less than 5% neurons. These results suggest that antigenic sites tubulin NF proteins are shared Different calf preparation, human-2 fractions contained a prominent protein band ferritin weight cross-reacted anti-human-2 anti-human-ferritin serums. However, this ferritinlike protein, or anti-ferritin, stain Although neither 2 nor two other human failed elicit antiserum these able remove activity binds data (or proteins) makes up is present various quantities microtubule brain.