A simple method for quantitative determination of polysaccharides in fungal cell walls
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摘要: A simple and reliable method for quantitative determination of cell wall polymers in fungal with an s.e.m. 5% is described. This protocol based on the hydrolysis by sulfuric acid beta-glucan, mannan, galactomannan chitin present at different levels yeasts filamentous fungi into their corresponding monomers glucose, mannose, galactose glucosamine. The released monosaccharides are subsequently separated quantified high-performance ionic chromatography coupled to pulse amperometry detection, a detection limit 1.0 mug ml(-1). procedure well suited screening large collection yeast mutants or evaluating effects environmental conditions polysaccharide content. also applicable other species, including Schizosaccharomyces pombe, Candida albicans Aspergillus fumigatus. Results can be obtained 3 d.
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