Differential effects of viral hemorrhagic septicaemia virus (VHSV) genotypes IVa and IVb on gill epithelial and spleen macrophage cell lines from rainbow trout (Oncorhynchus mykiss)
作者： P.H. Pham , J.S. Lumsden , C. Tafalla , B. Dixon , N.C. Bols
DOI: 10.1016/J.FSI.2012.12.004
关键词:
摘要: The two most prominent genotypes of viral hemorrhagic septicemia virus (VHSV) are -I in the Northeastern Atlantic region and -IV North America, but much more is known about cellular pathogenesis genotype than -IV. VHSV divided into -IVa from Northeast Pacific Ocean -IVb Great Lakes both which less virulent to rainbow trout -I. In this work, infections VHSV-IVa have been studied cell lines, RTgill-W1 gill epithelium, RTS11 spleen macrophages. produced infectious progeny -IVb. However, was toward RTgill-W1: caused cytopathic effect (CPE) at a lower titre, elicited CPE earlier, yielded higher titres. By contrast, no increase titre were observed cultures infected with either genotype. Yet all six genes expressed antiviral genes, Mx2 Mx3, up regulated by VHSV-IVb -IVa. replication appeared terminate translational stage as N protein, presumably abundant VSHV proteins, not detected cultures. RTgill-W1, Mx3 similar levels genotypes, while induced IFN1, IFN2 LGP2A VHSV-IVb.
elsevier.com
sci-hub.se 参考文章(45)
T Gadd, M Jakava-Viljanen, H Tapiovaara, P Koski, L Sihvonen, Epidemiological aspects of viral haemorrhagic septicaemia virus genotype II isolated from Baltic herring, Clupea harengus membras L. Journal of Fish Diseases. ,vol. 34, pp. 517- 529 ,(2011) , 10.1111/J.1365-2761.2011.01264.X
J W Park, C H Moon, A R Wargo, M K Purcell, G Kurath, Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout-derived cell line, RTG-2. Journal of Fish Diseases. ,vol. 33, pp. 583- 591 ,(2010) , 10.1111/J.1365-2761.2010.01153.X
J W Park, C H Moon, A Harmache, A R Wargo, M K Purcell, M Bremont, G Kurath, Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity Journal of Fish Diseases. ,vol. 34, pp. 115- 129 ,(2011) , 10.1111/J.1365-2761.2010.01225.X
Jun Zou, Carolina Tafalla, Jonathan Truckle, Chris J. Secombes, Identification of a second group of type I IFNs in fish sheds light on IFN evolution in vertebrates. Journal of Immunology. ,vol. 179, pp. 3859- 3871 ,(2007) , 10.4049/JIMMUNOL.179.6.3859
Carolina Tafalla, Esther Sanchez, Niels Lorenzen, Stephanie J. DeWitte-Orr, Niels C. Bols, Effects of viral hemorrhagic septicemia virus (VHSV) on the rainbow trout (Oncorhynchus mykiss) monocyte cell line RTS-11. Molecular Immunology. ,vol. 45, pp. 1439- 1448 ,(2008) , 10.1016/J.MOLIMM.2007.08.015
Katja Einer-Jensen, Peter Ahrens, Roald Forsberg, Niels Lorenzen, Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus. Journal of General Virology. ,vol. 85, pp. 1167- 1179 ,(2004) , 10.1099/VIR.0.79820-0
G. Kärber, BEITRAG ZUR KOLLEKTIVEN BEHANDLUNG PHARMAKOLOGISCHER REIHENVERSUCHE Naunyn-schmiedebergs Archives of Pharmacology. ,vol. 162, pp. 480- 483 ,(1931) , 10.1007/BF01863914
JS Lumsden, B Morrison, C Yason, S Russell, K Young, A Yazdanpanah, P Huber, L Al-Hussinee, D Stone, K Way, Mortality event in freshwater drum Aplodinotus grunniens from Lake Ontario, Canada, associated with viral haemorrhagic septicemia virus, type IV Diseases of Aquatic Organisms. ,vol. 76, pp. 99- 111 ,(2007) , 10.3354/DAO076099
Phuc H. Pham, Junwon Jung, Niels C. Bols, Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces Cytotechnology. ,vol. 63, pp. 385- 397 ,(2011) , 10.1007/S10616-011-9355-8
A. ESTEPA, D. FRÍAS, J.M. COLL, Susceptibility of trout kidney macrophages to viral hemorrhagic septicemia virus. Viral Immunology. ,vol. 5, pp. 283- 292 ,(1992) , 10.1089/VIM.1992.5.283