Design and analysis of metabolic pathways supporting formatotrophic growth for electricity-dependent cultivation of microbes.

摘要: Electrosynthesis is a promising approach that enables the biological production of commodities, like fuels and fine chemicals, using renewably produced electricity. Several techniques have been proposed to mediate transfer electrons from cathode living cells. Of these, electroproduction formate as mediator seems especially promising: readily soluble, low toxicity can be at relatively high efficiency reasonable current density. While organisms are capable formatotrophic growth, i.e. growth on formate, exist naturally, they generally less suitable for bulk cultivation industrial needs. Hence, it may helpful engineer model organism relevance, such E. coli, formate. There numerous metabolic pathways potentially support growth. Here we analyze these diverse according various criteria including biomass yield, thermodynamic favorability, chemical motive force, kinetics practical challenges posed by their expression. We find reductive glycine pathway, composed tetrahydrofolate system, cleavage serine hydroxymethyltransferase deaminase, candidate electrosynthesis in coli. The presented here exemplifies how combining different computational approaches into systematic analysis methodology provides assistance redesigning metabolism. This article part Special Issue entitled: Metals Bioenergetics Biomimetics Systems.