Prediction, mapping and validation of tick glutathione S-transferase B-cell epitopes.

摘要: In search of ways to address the increasing incidence global acaricide resistance, tick control through vaccination is regarded as a sustainable alternative approach. Recently, novel cocktail antigen tick-vaccine was developed based on recombinant glutathione S-transferase (rGST) anti-sera cross-reaction S-transferases Rhipicephalus appendiculatus (GST-Ra), Amblyomma variegatum (GST-Av), Haemaphysalis longicornis (GST-Hl), decoloratus (GST-Rd) and microplus (GST-Rm). Therefore, current study aimed predict shared B-cell epitopes within GST sequences these species. Prediction proteasomal cleavage sites were performed using immunoinformatics algorithms. The conserved predicted mapped homodimers respective GSTs, corresponding peptides independently used for rabbit immunization experiments. Based dot blot assay, immunogenicity their potential be recognized by rGST raised in previous work investigated. This revealed that five localized surface homodimers. GST-Ra, GST-Rd, GST-Av, GST-Hl also shown contain seven residue-long peptide sequence with no sites, whereas digestion GST-Rm yield 4-residue fragment. Given few found epitope four could T-cell epitope. Finally, reacted against peptide, confirming immunogenicity. These data support claim rGSTs, study, epitopes, which elucidates why cross-reacted non-homologous GSTs. Taken together, suggest this useful constituting epitope-based vaccines.