Catalytic behavior and detoxifying ability of a laccase from the fungal strain Cerrena unicolor
作者： L. Gianfreda , F. Sannino , M.T. Filazzola , A. Leonowicz
DOI: 10.1016/S1381-1177(97)00016-7
关键词:
摘要: The kinetics and stability of a laccase isolated purified from the fungal strain Cerrena unicolor were studied. enzyme was produced in great yield without inducers. Kinetic parameters determined by using 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) as substrate. At high ABTS concentrations (> 10 mM) substrate inhibition phenomenon appeared an constant Ki 24 mM determined. pH- temperature-profiles well sensitivity to several deactivation agents almost similar those observed with different origins. Freezing-thawing treatment, temperature, acidic pH (< 3.0) acetonitrile strongly affected activity. showed good ability oxidize phenolic substances; significant enhancing effect acting co-substrate. These results seem suggest that this new preparation may be suitable for environmental purposes.
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sci-hub.se 参考文章(24)
Robert Bourbonnais, Michael G. Paice, Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Letters. ,vol. 267, pp. 99- 102 ,(1990) , 10.1016/0014-5793(90)80298-W
Jean-Marc Bollag, Andrzej Leonowicz, Comparative studies of extracellular fungal laccases. Applied and Environmental Microbiology. ,vol. 48, pp. 849- 854 ,(1984) , 10.1128/AEM.48.4.849-854.1984
G. Palmeiri, P. Giardina, L. Marzullo, B. Desiderio, G. Nittii, R. Cannio, G. Sannia, Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus. Applied Microbiology and Biotechnology. ,vol. 39, pp. 632- 636 ,(1993) , 10.1007/BF00205066
Oleg Milstein, Aloys Hüttermann, Andrzej Majcherczyk, Kerstin Schulze, Rüdiger Fründ, Hans-Dietrich Lüdemann, Transformation of lignin-related compounds with laccase in organic solvents Journal of Biotechnology. ,vol. 30, pp. 37- 48 ,(1993) , 10.1016/0168-1656(93)90025-I
Robert W. Lencki, Joseph Arul, Ronald J. Neufeld, Effect of subunit dissociation, denaturation, aggregation, coagulation, and decomposition on enzyme inactivation kinetics: I. First-order behaviour. Biotechnology and Bioengineering. ,vol. 40, pp. 1421- 1426 ,(1992) , 10.1002/BIT.260401117
Jean-Marc Bollag, Jawed M. Sarkar, Andrzej Leonowicz, Improvement in stability of an immobilized fungal laccase Applied Microbiology and Biotechnology. ,vol. 29, pp. 129- 135 ,(1988) , 10.1007/BF01982891
C. F. Thurston, The structure and function of fungal laccases Microbiology. ,vol. 140, pp. 19- 26 ,(1994) , 10.1099/13500872-140-1-19
Jawed M. Sarkar, Andrzej Leonowicz, Jean-Marc Bollag, Immobilization of enzymes on clays and soils Soil Biology and Biochemistry. ,vol. 21, pp. 223- 230 ,(1989) , 10.1016/0038-0717(89)90098-9
L. Roy-Arcand, F.S. Archibald, Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor Enzyme and Microbial Technology. ,vol. 13, pp. 194- 203 ,(1991) , 10.1016/0141-0229(91)90128-W
Robert W. Lencki, Joseph Arul, Ronald J. Neufeld, Effect of subunit dissociation, denaturation, aggregation, coagulation, and decomposition on enzyme inactivation kinetics: II. Biphasic and grace period behavior. Biotechnology and Bioengineering. ,vol. 40, pp. 1427- 1434 ,(1992) , 10.1002/BIT.260401118