Expression of a cascading genetic network within liposomes
作者： Keitaro Ishikawa , Kanetomo Sato , Yasufumi Shima , Itaru Urabe , Tetsuya Yomo
DOI: 10.1016/J.FEBSLET.2004.09.046
关键词:
摘要: Liposomes have long been used as possible compartments for artificial cells, and it has shown that liposomes can sustain various types of biochemical reactions. To elevate the degree molecular complexity system in liposomes, we constructed a two-stage genetic network encapsulated liposomes. This was with plasmid pTH, which protein product first stage (T7 RNA polymerase) is required to drive synthesis second (GFP). We show cell-free expression functional within
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