Characterization of a nonfimbrial mannose-sensitive hemagglutinin (MSH) produced by Salmonella enterica serovar enteritidis.

作者: Jane M.G. Mikcha , Maria G. Freire , Maria Ligia R. Macedo , Tomomasa Yano , Antonio J. Piantino Ferreira

DOI: 10.1016/J.CIMID.2006.08.003

关键词:

摘要: Abstract A nonfimbrial mannose-sensitive hemagglutinin (MSH) with adhesive properties produced by Salmonella enterica serovar Enteritidis was characterized. The MSH characterized as glycoprotein and consisted of three noncovalently bound subunits M r 28, 33 40 kDa determined SDS-PAGE. heat-stable resistant to alkaline (high) or acid (low) pH, however, it inhibited proteolytic enzymes, EDTA sodium periodate. Mouse antiserum raised against reacted the 28 kDa band in immunoblotting, also hemagglutination bacterial adherence HeLa cells. Electron microscope examinations showed that is not a fimbriae-like structure. anti-MSH IgG competitively immunofluorescence test, using on cells specific IgG, supported view contributes organism. These results indicate putative factor may mediate enteritidis eucaryotic

参考文章(36)
N. Gilboa-Garber, D. Avichezer, N. C. Garber, Bacterial Lectins: Properties, Structure, Effects, Function and Applications Wiley‐VCH Verlag GmbH. pp. 369- 396 ,(2008) , 10.1002/9783527614738.CH21
V L Sheladia, D J Evans, J Guevara, J P Chambers, Isolation, purification, and partial characterization of type V-A hemagglutinin from Escherichia coli GV-12, O1:H-. Journal of Bacteriology. ,vol. 152, pp. 757- 761 ,(1982) , 10.1128/JB.152.2.757-761.1982
Stanley W.B. Ewen, Patrick J. Naughton, George Grant, Marcjanna Sojka, Emma Allen-Vercoe, Susan Bardocz, Christopher J. Thorns, Arpad Pusztai, Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo. Fems Immunology and Medical Microbiology. ,vol. 18, pp. 185- 192 ,(1997) , 10.1111/J.1574-695X.1997.TB01044.X
D. C. Rodrigue, R. V. Tauxe, B. Rowe, International increase in Salmonella enteritidis: a new pandemic? Epidemiology and Infection. ,vol. 105, pp. 21- 27 ,(1990) , 10.1017/S0950268800047609
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Heike Stolpe, Siegfried Grund, Werner Schroder, Purification and partial characterization of type 3 fimbriae from Salmonella typhimurium var. copenhagen Zentralblatt für Bakteriologie. ,vol. 281, pp. 8- 15 ,(1994) , 10.1016/S0934-8840(11)80631-6
Per Klemm, Mark A. Schembri, Bacterial adhesins: function and structure. International Journal of Medical Microbiology. ,vol. 290, pp. 27- 35 ,(2000) , 10.1016/S1438-4221(00)80102-2
Z.P. Camargo, C.P. Taborda, E.G. Rodrigues, L.R. Travassos, The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests Medical Mycology. ,vol. 29, pp. 31- 38 ,(1991) , 10.1080/02681219180000061
R. A. Edwards, D. M. Schifferli, S. R. Maloy, A role for Salmonella fimbriae in intraperitoneal infections. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 1258- 1262 ,(2000) , 10.1073/PNAS.97.3.1258