Serum-free Culture of Chicken Bursal Epithelial Cells.
作者： Song Han , Eiichiro Mitsusada , Asaki Abe , Yasuhiro Kondo
DOI: 10.2141/JPSA.40.69
关键词:
摘要: We developed a method of culturing bursal epithelial cells under serum-free condition. Bursal sampled from 14 days embryogenesis were cultured in DMEM/Ham’s F12 1 : mixture medium supplemented with epidermal growth factor, insulin, transferrin, sodium selenite, hydrocortisone and 2-aminoethanol. A monolayer, which polygonal extensively observed, entirely covered the well after 2 to 3 weeks incubation. The maintained for 5 months without any apparent morphological abnormalities. Polygonal wells or reacted anti-keratin antibody, indicating these are cytokeratin-positive cells. These results suggest that most proliferated condition It seems possible precisely analyze presumable factors supernatant cell culture.
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