Enhanced gene transfer into brain capillary endothelial cells using Antp-modified DNA-loaded nanoparticles.
作者： Rong-qin Huang , Yuan-ying Pei , Chen Jiang
DOI: 10.1007/S11373-007-9171-5
关键词:
摘要: Brain capillary endothelial cells (BCECs) have been considered as one of the primary targets for cerebral gene therapy. However, cells, well-known their poor function endocytosis, are difficult to be transfected by general non-viral vectors. The aim this study was enhance efficiency transfection and expression in BCECs DNA/polymer nanoparticles with modification membrane-penetrating peptide, Antennapedia peptide (Antp) polyethylenimine (PEI) polyamidoamine (PAMAM) were chosen prepare Antp-modified DNA-loaded a complex coacervation technique. After 20-min transfection, efficiency, terms expression, DNA/PEI NP or DNA/PAMAM enhanced significantly Antp. 3-h DNA/Antp/PEI NP, there no difference cellular uptake but an enhancement compared alone. both using These observations suggest that Antp can increase ability nanoparticles, which employed novel
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