Optical fiber-based in vivo quantification of growth factor receptors

摘要: BACKGROUND: Growth factor receptors such as epidermal growth receptor 1 and human 2 (HER2) are overexpressed in certain cancer cells. Antibodies against these (eg. cetuximab transtuzumab [Herceptin]) have shown therapeutic value treatment. The existing methods for the quantification of tumors involve immunohistochemistry or DNA quantification, both extracted tissue samples. goal study was to evaluate whether an optical fiber-based technique can be used quantify expression multiple simultaneously. METHODS: The authors examined HER2 using monoclonal antibody trastuzumab a targeting ligand test their system. They conjugated different Alexa Fluor dyes with excitation emission wavelengths. Two dye conjugates were subsequently injected intravenously into mice bearing HER2-expressing subcutaneous tumors. An fiber then inserted tumor through 30-gauge needle, single laser beam source, fluorescence emitted by identified quantified 2-photon fluorescence. RESULTS: The bound competitively receptor-specific fashion, but they failed bind similar cell that did not express HER2. concentration conjugate present determined serve index levels. CONCLUSIONS: These studies offer minimally invasive simultaneously. Cancer 2012;. © 2011 American Society.