Effects of exogenous peripheral-blood-derived endothelial progenitor cells or unfractionated bone-marrow-derived cells on neointimal formation and inflammation in cholesterol-fed, balloon-denuded, and radiated iliac arteries of inbred rabbits

摘要: Abstract Background Injection of bone marrow cells (BMC) and endothelial progenitor (EPC) or application stem-cell-mobilizing factors has been associated both with reduction exacerbation atherosclerosis unstable plaque phenotype. The discrepancies may reflect the cell type, dosing, duration, route administration in these studies. aim this study was to determine effects peripheral-blood-derived (PBEPC) unfractionated BMC obtained from inbred siblings on neointimal formation inflammation cholesterol-fed, balloon-denuded, radiated rabbit iliac arteries. Methods Rabbits were fed a 1.0% cholesterol diet for 14 days, followed by denudation arteries, continued 0.15% diet. On day 42, denuded areas radiated, animals randomized. first group received PBEPC ( n =5), second =4), third heparinized (20 IU) saline (Control; =3). characterized flow cytometry. Cells (5×10 6 ) administered twice through ear vein: time at 1 h after radiation 2 weeks radiation. Four radiation, sacrificed, arterial segments processed morphometry. Results Administration had no adverse effect. Lumen area (0.93±0.53 mm ), (0.65±0.29 percent stenosis (44±21), macrophage score (0.6±0.3) controls similar those cell-treated groups. Conclusion This demonstrates that, current animal model, either failed affect inflammation.