Characterization of Beauveria bassiana neutral trehalase (BbNTH1) and recognition of crucial stress-responsive elements to control its expression in response to multiple stresses.

摘要: Abstract A neutral trehalase (NTH1) of fungal entomopathogen Beauveria bassiana was characterized for the first time as a 743-aa enzyme (84.4 kDa). To identify crucial stress-responsive elements (STREs) to control expression NTH-coding gene ( BbNTH1 ) in response different stresses, full-length promoter (−2713 bp) upstream its open reading frame and three upstream-truncated fragments (−1912, −1060 −560 bp) were fused reporter eGFP then transformed into B. , respectively. Consequently, well expressed intensive fluorescence mycelia, conidiogenic cells forming conidia controlled by with five STREs. Surprisingly, transformants shortest fragment last two STREs at −315 −274 bp exhibited consistently brightest mycelia under 3-h oxidative adaption 0.3–1.2 mM menadione, colonies 6-day osmotic stress 0.5–1 M NaCl thermal 15–540 min 40 °C after 3-day growth 25 °C. Single or dual site-directed mutations from C CC CT AT significantly altered multiple stresses. Thus, downstream 560-bp region are regulating not only constitutive but stress-inducible target gene.