Induction of neutral trehalase Nth1 by heat and osmotic stress is controlled by STRE elements and Msn2/Msn4 transcription factors: variations of PKA effect during stress and growth

摘要: Saccharomyces cerevisiae neutral trehalase, encoded by NTH1, controls trehalose hydrolysis in response to multiple stress conditions, including nutrient limitation. The presence of three responsive elements (STREs, CCCCT) the NTH1 promoter suggested that transcriptional activator proteins Msn2 and Msn4, as well cAMP-dependent protein kinase (PKA), control stress-induced expression Nth1. Here, we give direct evidence Msn2/Msn4 STREs heat-, osmotic stress- diauxic shift-dependent induction Disruption MSN2 MSN4 abolishes or significantly reduces heat- NaCl-induced increases Nth1 activity transcription. Stress-induced a lacZ reporter gene put under is nearly absent double mutant. In all instances, basal also reduced about 50%. concentration msn2 msn4 mutant less during heat drops more slowly recovery than wild-type cells. This shows Msn2/Msn4-controlled enzymes synthesis help maintain stress. However, Msn2/Msn4-independent mechanism exists for metabolism. Site-directed mutagenesis (CCCCT changed CATCT) fused indicates relative proximity each other important function NTH1. Elimination responses 30%. Contrary most STRE-regulated genes, PKA effect on sodium chloride variable. During growth, promoter-controlled strongly increases, opposed previously observed decrease activity, suggesting tight but opposite enzyme at post-translational levels. Apparently, inactive trehalase accumulated concomitant with accumulation trehalose. These results might elucidate general connection between STREs, and, particular, how these components play role