Yap1p, a yeast transcriptional activator that mediates multidrug resistance, regulates the metabolic stress response.

摘要: Overexpression of the YAP1 transcriptional activator renders yeast cells resistant to multiple metabolic inhibitors. In an effort identify other gene products required for this phenotype we have isolated genomic mutations which neutralize effect. One such mutation was further characterized and affected shown be identical TPS2 encodes trehalose phosphate phosphatase, enzyme catalysing second step in biosynthesis. We analysed regulation that its transcription is induced by a variety stressful conditions caused inhibitors, osmotic shock heat shock. This activation mediated stress promoter elements (C4T) requires function Yap1p as well reduced activity cAMP-regulated protein kinase. Using appropriate reporter generally through C4T element. These results show has pivotal role response acquisition tolerance.