Hair analysis method

摘要: A method for the direct analysis of analyte indicative marijuana exposure found in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing dithiothreitol or dithioerythritol, protease suitable digestion keratin structure sample structure; permitting enzyme to at least substantially digest form solution, followed by mixing solution with suspension an ion exchange resin remove interfering, cross reacting substance naturally hair finally subjecting determine identity amount sample. To accelerate method, cupric sulfate may be added after degradation order deactivate activator. The papain, chymopapain, proteinase K being preferred use invention. Exemplary resins useful according invention are DEAE Sephadex (Diethylaminoethyl Sephadex) Sepharose Sepharose).